Dr. Manpreet Kalkat completed her undergraduate and graduate studies at University of Toronto, where she earned a B.Sc. with distinction in human biology, an M.Sc. in physiology and a Ph.D. in medical biophysics with a focus on novel regulators of Myc activity. This work further elucidated Myc’s role in tumorigenesis and may lead to novel strategies for inhibiting Myc in cancer. Over the years, she has won numerous awards for research and scholarship, including the prestigious Canadian Breast Cancer Foundation Doctoral Fellowship. In 2017, she joined the laboratory of Dr. Peter Laird at Van Andel Research Institute as a postdoctoral fellow.
Dr. Kalkat studies the functional significance of DNA methylation states in models of colorectal cancer. She is using a novel reporter system to monitor loss of heterozygosity of the Apc gene in a mouse model of intestinal cancer and in ex vivo organoid culture systems.
Ph.D., Princess Margaret Cancer Center, University of Toronto (Adviser: Dr. Linda Z. Penn)
Thesis: Identifying novel regulators of Myc activity
M.Sc., University of Toronto (Adviser: Dr. Isabella Caniggia)
Thesis: Regulation of placental autophagy by the Bcl-2 family proteins Mcl-1 and Mtd
B.Sc. in human biology (conferred with distinction), University of Toronto
To view a list of selected publications click below.
Lustig LC, Dingar D, Tu WB, Lourenco C, Kalkat M, Ponzielli R, Inamoto I, Chan WCW, Shin JA, Penn LZ. In press. Inhibiting MYC binding to the E-box DNA motif by ME47 decreases tumour xenograft growth. Oncogene.
Kalkat M, De Melo J, Hickman KA, Lourenco C, Redel C, Resetca D, Tamachi A, Tu WB, Penn LZ. 2017. MYC deregulation in primary human cancers. Genes 8(6):151; doi:10.3390/genes8060151.
Pandyra AA, Mullen PJ, Goard CA, Ericson E, Sharma P, Kalkat M, Yu R, Pong JT, Brown KR, Hart T, Gebbia M, Lang KS, Giaever G, Nislow C, Moffat J, Penn LZ. 2015. Genome-wide RNAi analysis reveals that simultaneous inhibition of specific mevalonate pathway genes potentiates tumor cell death. Oncotarget 6(29):26909–26921.
Dingar D*, Kalkat M*, Chan PK, Srikumar T, Bailey SD, Tu WB, Coyaud E, Ponzielli R, Kolyar M, Jurisica I, Huang A, Lupien M, Penn LZ, Raught B. 2015. BioID identifies novel c-MYC interacting partners in cultured cells and xenograft tumors. J Proteomics 118: 95–111.
Chan PK, Srikumar T, Dingar D, Kalkat M, Penn LZ, Raught B. 2014. BioID data of c-MYC interacting protein partners in cultured cells and xenograft tumors. Data Brief 1:76–78.
Kalkat M, Chan PK, Wasylishen AR, Srikumar T, Kim SS, Ponzielli R, Bazett-Jones DP, Raught B, Penn LZ. 2014. Identification of c-MYC SUMOylation by mass spectrometry. PloS One 9:e115337.
Pandyra A, Mullen PJ, Kalkat M, Yu R, Pong JT, Li Z, Trudel S, Lang KS, Minden MD, Schimmer AD, Penn LZ. 2014. Immediate utility of two approved agents to target both the metabolic mevalonate pathway and its restorative feedback loop. Cancer Res 74:4772–4782.
Wasylishen AR, Kalkat M, Kim SS, Pandyra A, Chan PK, Oliveri S, Sedivy E, Konforte D, Bros C, Raught B, Penn LZ. 2014. MYC activity is negatively regulated by a C-terminal lysine cluster. Oncogene 33(8):1066–1072.
Kalkat M, Penn LZ. Regulation and function of the MYC oncogene. In: Encyclopedia of Life Sciences (eLS). John Wiley & Sons Ltd, Chichester. 2013.
Wasylishen AR, Chan-Seng-Yue M, Bros C, Dingar D, Tu WB, Kalkat M, Chan P-K, Mullen PJ, Huang L, Meyer N, Raught B, Boutros PC, Penn LZ. 2013. MYC phosphorylation at novel regulatory regions suppresses transforming activity. Cancer Res 73:6504-6515.
Kalkat M, Garcia J, Ebrahimi J, Melland-Smith M, Todros T, Post M, Caniggia I. 2013. Placental autophagy regulation by the BOK-MCL1 rheostat. Autophagy 9:2140–2153.
Kalkat M, Wasylishen AR, Kim SS, Penn L. 2011. More than MAX: Discovering the Myc interactome. Cell Cycle 10(3):374-379.