Dr. Feng Wang has his Ph.D. in microbiology and a B.S. in biotechnology from Shandong University. His graduate work focused on the secondary messenger regulation in pathogens, such as the critical virulence factor pyocyanin and intracellular cyclic dinucleotide (c-di-GMP and c-di-AMP). Dr. Wang’s lab skills include X-ray crystallography, Protein expression and protein glycosylation. In 2020, he joined the lab of Dr. Huilin Li as a postdoctoral fellow.
Origin recognition complex (ORC), which consists of Orc1-Orc6, directs eukaryotic DNA replication and remains bound to chromatin at replication origins throughout the cell cycle. While ORC is only active in late mitosis and early G1 phase, it has been reported that the cyclin-dependent protein kinase (CDK) could phosphorylate ORC to regulate initiation of DNA replication. CDK could interact with ORC and regulate phosphorylation of Orc2 and Orc6. However, how phosphorylation of Orc2 and Orc6 regulate the DNA binding activity of ORC complex is still unclear. We want to use cryo-EM to solve the structures of ORC and CDK complex or phosphorylated ORC and DNA complex, combined with some biochemical experiments to generate a regulation mechanism for phosphorylated ORC and helicase loading.
Ph.D. in microbiology, Shandong University
Thesis: BrlR from Pseudomonas aeruginosa is a common receptor for both cyclic di-GMP and pyocyanin (Advisor: Lichuan Gu, Ph.D.)
B.S. in biotechnology, Shandong University
To view a list of selected publications click below.
Wang F*, He Q*, Yin J, Xu S, Hu W, Gu L. 2018. BrlR from Pseudomonas aeruginosa is a common receptor for both cyclic di-GMP and pyocyanin. Nat Commun 2(9):2563
Wang F*, He Q*, Su K*, Wei T, Xu S, Gu L. 2017. Structural and biochemical characterization of the catalytic domains of GdpP reveals a unified hydrolysis mechanism for the DHH-DHHA1 domain. Biochem J 475(1):191–205.
Wang F*, He Q*, Su K, Gao F, Huang Y, Lin Z, Zhu D, Gu L. 2016. The PilZ domain of MrkH represents a novel DNA binding motif. Protein Cell 7(10):766–772.
He Q*, Wang F*, Liu S, Zhu D, Cong H, Gao F, Li B, Wang H, Lin Z, Liao J, Gu L. 2016. Structural and Biochemical Insight into the Mechanism of Rv2837c from Mycobacterium tuberculosis as a c-di-NMP Phosphodiesterase. J Biol Chem 291(7):3668–3681.
Li B, Li N, Wang F, Guo L, Huang Y, Liu X, Wei T, Zhu D, Liu C, Pan H, Xu S, Wang HW, Gu L. 2012. Structural insight of a concentration-dependent mechanism by which YdiV inhibits Escherichia coli flagellum biogenesis and motility. Nucleic Acids Res 40 (21):11073–11085.